Part:BBa_J45004:Design

SAM:benzoic acid/salicylic acid carboxyl methyltransferase I; converts salicylic acid to methyl sali

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 663
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 204
  

Design Notes

Constructed by PCR.

Template

pET-28a-BSMT1Negre03

• We thank Natalia Dudareva, Department of Horticulture and Landscape Architecture, Purdue University for the gift of the expression vector with the petunia BSMT1 coding sequence.

Forward primer

5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GAT GGA AGT TGT TGA AGT TC -3'

Reverse primer

5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAA TTT ATT TTG GTC AAG GAG -3'

Source

• BSMT1 gene from Petunia x hybrida (GenBank [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&val=28629494 AY233465])

References

<biblio>

1. Underwood05 pmid=15849311
2. Negre03 pmid=14630969

</biblio>